Question
Nature Methods crowned this paradigm that combines imaging and genomics 2020’s Method of the Year. For 10 points each:
[10h] Name this family of methods that, unlike sc-RNA-seq (“S-C-RNA-seek”), doesn’t require liberating cells from tissue and can thus analyze single cells in their native contexts. 10x’s Visium is a commercially available technique in this family.
ANSWER: spatial transcriptomics [or spatially-resolved transcriptomics; accept, but DO NOT REVEAL, in situ transcriptomics; prompt on transcriptomics or transcriptome profiling]
[10m] A spatial transcriptomics method uses a multiplexed, error-robust version of this technique with binary labels to measure RNA species in tissue. A quantitative version of this technique can measure telomere lengths.
ANSWER: fluorescent in situ hybridization [or FISH; accept MERFISH; accept Q-FISH]
[10e] A landmark spatial profiling paper by Zhuang et al. mapped the preoptic nucleus of this brain structure, which controls the pituitary gland, hunger, thirst, and other autonomic processes.
ANSWER: hypothalamus [accept hypothalamic–pituitary–adrenal axis; prompt on HPA axis]
Data
| Team | Opponent | Part 1 | Part 2 | Part 3 | Total |
|---|---|---|---|---|---|
| Chicago B | Johns Hopkins A | 0 | 0 | 10 | 10 |
| Florida A | Toronto A | 0 | 0 | 10 | 10 |
| Georgia Tech A | MIT A | 0 | 0 | 0 | 0 |
| Iowa State A | North Carolina A | 0 | 0 | 0 | 0 |
| Maryland A | Harvard A | 0 | 0 | 0 | 0 |
| Yale A | Ohio State A | 0 | 0 | 10 | 10 |