Question

Each heavy chain of this heterotetrameric protein consists of a head connected by a “neck linker” to a stalk ending in a C-terminal tail. For 10 points each:
[10m] Name this protein family whose “walking” motion has been proposed to proceed via either a “hand-over-hand” or “inchworm” mechanism.
ANSWER: kinesins [prompt on motor proteins; accept kinesin motor domain superfamily]
[10e] Experiments to determine the true mechanism of “walking” kinesin used constructs with solvent-exposed residues mutated to this amino acid, whose sulfhydryl group can be conjugated to Cy3 (“sigh three”) or Cy5 (“sigh five”).
ANSWER: cysteine [or Cys; or C]
[10h] Cy3 and Cy5 are a popular pair of dyes for this nanometer-scale technique. Rice et al.’s paper suggesting hand-over-hand motion used this technique to demonstrate the kinesin neck linker’s motion when kinesin binds ATP.
ANSWER: smFRET [or single-molecule fluorescence resonance energy transfer; or single-molecule Förster resonance energy transfer; prompt on FRET or fluorescence resonance energy transfer or Förster resonance energy transfer

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Data

TeamOpponentPart 1Part 2Part 3Total
Chicago AOhio State A010010
Chicago BDuke A1010020
Columbia AHarvard A0000
Cornell AStanford A1010020
Florida AYale A0000
Florida BJohns Hopkins A0000
Georgia Tech AMaryland A010010
Imperial ANYU A010010
Iowa State AClaremont A0000
Minnesota AMIT A0000
North Carolina AChicago C010010
Penn AIllinois A010010
Penn State AVirginia A100010
Purdue AMinnesota B1010020
Rutgers AWUSTL B1010020
Texas AIndiana A100010
Toronto ACornell B1010020
UC Berkeley ANorthwestern A010010
UC Berkeley BVanderbilt A10101030
WUSTL ABrown A010010